5803 thp 1 atcc cat Search Results


5803 thp 1 atcc cat  (ATCC)
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ATCC 5803 thp 1 atcc cat
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293t/17  (ATCC)
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ATCC 293t/17
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thp-1  (ATCC)
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ATCC thp-1
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anti-myc ab–conjugated agarose beads  (Millipore)
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anti-ha ab–conjugated agarose beads  (Millipore)
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Millipore anti-ha ab–conjugated agarose beads
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anti-flag ab–conjugated agarose beads  (Millipore)
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Millipore anti-flag ab–conjugated agarose beads
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fg4592 selleck cat  (Selleck Chemicals)
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Selleck Chemicals fg4592 selleck cat
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piclv dmso mp biomedicals  (Valiant Co Ltd)
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Valiant Co Ltd piclv dmso mp biomedicals
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fluorescein conjugated thermo fisher scientific cat  (Thermo Fisher)
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Thermo Fisher fluorescein conjugated thermo fisher scientific cat
KEY RESOURCES TABLE
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concanavalin a  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc concanavalin a
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stop solution  (Cell Signaling Technology Inc)
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p36931 tmb substrate cell signaling technology  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc p36931 tmb substrate cell signaling technology
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Image Search Results


KEY RESOURCES TABLE

Journal: Cancer cell

Article Title: Removal of N-linked glycosylation enhances PD-L1 detection and predicts anti-PD-1/PD-L1 therapeutic efficacy

doi: 10.1016/j.ccell.2019.06.008

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PD-L1 antibody (clone 28-8 mAb) Abcam Cat#ab205921; RRID:AB_2687878 CD274 rabbit polyclonal antibody Abcam Cat#ab58810; RRID:AB_940872 PD-L1 (E1L3N) XP rabbit antibody Cell Signaling Technology Cat#13684; RRID:AB_2687655 Atezolizumab MDACC N/A α-tubulin antibody (monoclonal) Sigma-Aldrich Cat#T5168; RRID:AB_477579 Actin antibody Sigma-Aldrich Cat#A2066; RRID:AB_476693 Peroxidase-AffiniPure Goat anti-Mouse IgG (H+L) Jackson ImmunoResearch Cat#115-035-003; RRID:AB_10015289 Peroxidase-AffiniPure Goat anti-Rabbit IgG (H+L) Jackson ImmunoResearch Cat#111-035-003; RRID:AB_2313567 Goat Anti-Rabbit IgG (H+L) antibody, Fluorescein Conjugated Thermo Fisher Scientific Cat#F2765; RRID:AB_10562896 Bacterial and Virus Strains N/A Biological Samples Multi-organ carcinoma tissue array Biomax Cat# BC000119 Lung cancer tissue array Biomax Cat#NSC151 Rectal cancer tissue array This paper N/A Human pre-immunotherapy archived tissue samples This paper N/A Chemicals, Peptides, and Recombinant Proteins PNGase F New England BioLabs Cat#P0704 AEC chromogen substrate (3-amino-9-ethylcarbazole) Sigma-Aldrich Cat#A5754 N,N -dimethylformamide Sigma-Aldrich Cat#D4254 Aqua-mount mounting medium DAKO Cat#S1964 ProLong™ Gold Antifade Mountant with DAPI Invitrogen Cat# P36931 TMB Substrate Cell Signaling Technology Cat#7004 TMB High Sensitivity Substrate Solution BioLegend Cat#421501 STOP Solution Cell Signaling Technology Cat#7002 Stop Solution for TMB Substrate BioLegend Cat#423001 BSA blocking solution for ELISA Santa Cruz Biotechnology Cat#293965 Animal-Free Blocking Solution Cell Signaling Technology Cat#15019 Concanavalin A, HRP-conjugated Sigma-Aldrich Cat#L6397 Critical Commercial Assays N/A Deposited Data N/A Experimental Models: Cell Lines BT-549 ATCC ATCC#HTB-122 BT-20 ATCC ATCC#HTB-19 MDA-MB-231 ATCC ATCC#HTB-26 MCF-7 ATCC ATCC#HTB-22 H1437 ATCC ATCC#CRL-5872 A549 ATCC ATCC#CCL-185 Calu3 ATCC ATCC#HTB-55 H1299 ATCC ATCC#CRL-5803 H1355 ATCC ATCC#CRL-5865 H358 ATCC ATCC#CRL-5807 H1435 ATCC ATCC#CRL-5870 H226 ATCC ATCC#CRL-5826 H322 ATCC ATCC#CRL-5806 Jurkat ATCC ATCC#TIB-152 THP1 ATCC ATCC#TIB-202 Experimental Models: Organisms/Strains N/A Oligonucleotides N/A Recombinant DNA N/A Software and Algorithms Prism (version 7) GraphPad N/A Other N/A Open in a separate window KEY RESOURCES TABLE Heavy glycosylation of PD-L1 hinders its detection by anti-PD-L1 antibodies and could lead to inaccurate readout from a variety of bioassays.

Techniques: Recombinant, Blocking Assay, Enzyme-linked Immunosorbent Assay, Software

KEY RESOURCES TABLE

Journal: Cancer cell

Article Title: Removal of N-linked glycosylation enhances PD-L1 detection and predicts anti-PD-1/PD-L1 therapeutic efficacy

doi: 10.1016/j.ccell.2019.06.008

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PD-L1 antibody (clone 28-8 mAb) Abcam Cat#ab205921; RRID:AB_2687878 CD274 rabbit polyclonal antibody Abcam Cat#ab58810; RRID:AB_940872 PD-L1 (E1L3N) XP rabbit antibody Cell Signaling Technology Cat#13684; RRID:AB_2687655 Atezolizumab MDACC N/A α-tubulin antibody (monoclonal) Sigma-Aldrich Cat#T5168; RRID:AB_477579 Actin antibody Sigma-Aldrich Cat#A2066; RRID:AB_476693 Peroxidase-AffiniPure Goat anti-Mouse IgG (H+L) Jackson ImmunoResearch Cat#115-035-003; RRID:AB_10015289 Peroxidase-AffiniPure Goat anti-Rabbit IgG (H+L) Jackson ImmunoResearch Cat#111-035-003; RRID:AB_2313567 Goat Anti-Rabbit IgG (H+L) antibody, Fluorescein Conjugated Thermo Fisher Scientific Cat#F2765; RRID:AB_10562896 Bacterial and Virus Strains N/A Biological Samples Multi-organ carcinoma tissue array Biomax Cat# BC000119 Lung cancer tissue array Biomax Cat#NSC151 Rectal cancer tissue array This paper N/A Human pre-immunotherapy archived tissue samples This paper N/A Chemicals, Peptides, and Recombinant Proteins PNGase F New England BioLabs Cat#P0704 AEC chromogen substrate (3-amino-9-ethylcarbazole) Sigma-Aldrich Cat#A5754 N,N -dimethylformamide Sigma-Aldrich Cat#D4254 Aqua-mount mounting medium DAKO Cat#S1964 ProLong™ Gold Antifade Mountant with DAPI Invitrogen Cat# P36931 TMB Substrate Cell Signaling Technology Cat#7004 TMB High Sensitivity Substrate Solution BioLegend Cat#421501 STOP Solution Cell Signaling Technology Cat#7002 Stop Solution for TMB Substrate BioLegend Cat#423001 BSA blocking solution for ELISA Santa Cruz Biotechnology Cat#293965 Animal-Free Blocking Solution Cell Signaling Technology Cat#15019 Concanavalin A, HRP-conjugated Sigma-Aldrich Cat#L6397 Critical Commercial Assays N/A Deposited Data N/A Experimental Models: Cell Lines BT-549 ATCC ATCC#HTB-122 BT-20 ATCC ATCC#HTB-19 MDA-MB-231 ATCC ATCC#HTB-26 MCF-7 ATCC ATCC#HTB-22 H1437 ATCC ATCC#CRL-5872 A549 ATCC ATCC#CCL-185 Calu3 ATCC ATCC#HTB-55 H1299 ATCC ATCC#CRL-5803 H1355 ATCC ATCC#CRL-5865 H358 ATCC ATCC#CRL-5807 H1435 ATCC ATCC#CRL-5870 H226 ATCC ATCC#CRL-5826 H322 ATCC ATCC#CRL-5806 Jurkat ATCC ATCC#TIB-152 THP1 ATCC ATCC#TIB-202 Experimental Models: Organisms/Strains N/A Oligonucleotides N/A Recombinant DNA N/A Software and Algorithms Prism (version 7) GraphPad N/A Other N/A Open in a separate window KEY RESOURCES TABLE Heavy glycosylation of PD-L1 hinders its detection by anti-PD-L1 antibodies and could lead to inaccurate readout from a variety of bioassays.

Techniques: Recombinant, Blocking Assay, Enzyme-linked Immunosorbent Assay, Software